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Calculate pi amino acid sequence2/25/2023 ![]() ![]() pI Charge isoelectric (SeqAA) returns the estimated isoelectric point ( pI) for an amino acid sequence and the estimated charge for a given pH (default is typical intracellular pH 7.2 ). You can access GPMAW lite free from any browser, computer, smartphone, or tablet at our website. The isoelectric point is the pH at which the protein has a net charge of zero. In conclusion, GPMAW lite is an online software for simple and easy bioinformatics calculations on protein amino acid sequences as an alternative to complete protein sequence analysis. Why use the GPMAW lite protein sequence calculator? The columns can thus show peptides by position, HPLC retention time, or mass. You do so by clicking the header of the column. ![]() You will also see the isoelectric point pI and expected retention time in an HPLC run (reversed phase) for each peptide. The table contains info about the peptide sequence and MH + (mono-isotopic molecular weight of the protonated peptide in reduced form). The protease Digest lists all peptides formed by complete theoretical digestion using the chosen protease. If the sequence contains any unusual amino acids, it will also be visible in this table. It includes numbers and percentages of individual amino acids. The Amino Acid Compositionfunction shows an amino acid composition table. It also shows the isoelectric point and hydrophobicity index. These include Molecular Weight (mono-isotopic and average mass of non-reduced protein), theoretically calculated molar absorbance, and extinction coefficient. The Protein Information table provides all the basic information about your protein. In addition, you can use the Colorize function to highlight specific amino acid residues and potential N-glycosylations sites. The protein’s name and sequence are shown at the top of the analysis results. First, select the analysis you would like to have completed. You can perform several different bioinformatics analyses when the protein sequence has been entered. It also works with spaces or lower case letters. The cleanup function is helpful if your sequence contains numbers. Or, you can type in the sequence using one letter AA code. Alternatively, paste the sequence directly into the protein sequence calculator window.ģ. With the NCBI and UniProt lookup function, you can easily find the accession number for any known protein from any organism.Ģ. The sequence will be retrieved into the sequence window automatically. There are three different ways to enter a sequence into GPMAW lite ġ. Either enter the known accession number from either NCBI or UNIPROT. Therefore, Alphalyse launched GPMAW lite bioinformatics tool to make these calculations quickly. What peptides does trypsin cleavage generate? ![]()
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